Thrombin from porcine blood | 凝血酶 来源于猪血

生物化学/生理学行为

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

分析注意事项

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

经常问的问题

Frequently Asked Questions are available for this Product.

制备注意事项

Activated with bovine brain thromboplastin.

物理形态

Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0

Lyophilized powder containing sodium chloride and Glycine

应用

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess persistent hypocoagulability in patients with septic shock.

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess persistent hypocoagulability in patients with septic sh

一般说明

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.